Journal: The Journal of infectious diseases

Article Title: Uropathogenic Escherichia coli alters muscle contractions in rat urinary bladder via a nitric oxide synthase-related signaling pathway.

doi: 10.1086/509620

Figure Lengend Snippet: Figure 1. Endothelial nitric oxide synthase (eNOS), protein kinase C (PKC), and extracellular signal-regulated kinase (ERK)–1 and –2 proteins in the urinary bladder 1 h after uropathogenic Escherichia coli (UPEC) treatment. Urinary bladders were isolated from rats 1 h after the intra- vesical instillation of E. coli strain J96 (O4:K6) or E. coli isolated from patients with acute E. coli pyelonephritis (EC). A, Representative eNOS and phosphorylated (p) eNOS Western blots of bladder after treatment with UPEC. The representative Western-blot images are shown (top and middle). A, bottom, Analysis of the relative amounts of peNOS protein in bladders treated with UPEC, relative to control. Data are the mean SE ( ). * vs. the control group. B and C, Activation of ERK1/ n p 5 P ! .05 2 and PKC studied by Western-blot analysis using anti-phospho ERK1/2 (B) and anti–phospho-PKC (pan) (C) antibodies, respectively. In some ex- periments of protein phosphorylation, okadaic acid (1 mmol/L), a serine/ threonine phosphatase inhibitor, was added to Western-blot samples (A, middle; B, bottom; and C, bottom). a-tubulin, served as the control for sample loading and integrity, and total ERK1/2 proteins were also shown. In panels B and C, results shown are representative of at least 4 experiments.

Article Snippet: The human pyelonephritogenic E. coli strain J96 serotype O4:K6 (ATCC 700336), which expresses type 1 and P-fimbrial adhesions, was used.

Techniques: Isolation, Western Blot, Control, Activation Assay, Phospho-proteomics

Journal: The Journal of infectious diseases

Article Title: Uropathogenic Escherichia coli alters muscle contractions in rat urinary bladder via a nitric oxide synthase-related signaling pathway.

doi: 10.1086/509620

Figure Lengend Snippet: Figure 2. Muscle contractions evoked by electrical field stimulation (EFS) or exogenous acetylcholine (ACh) in the urinary bladder 1 h after uropathogenic Escherichia coli (UPEC) treatment. Detrusor muscle strips isolated from rats 1 h after the intravesical instillation of J96 or E. coli isolated from patients with acute E. coli pyelonephritis (EC) were con- tracted by EFS (10 s at 4 and 16 Hz) (A) or ACh (B). In some experiments, rats were instilled intravesically with N G-nitro-L-arginine methyl ester (L- NAME) 30 min after the instillation of UPEC (C). Data are the mean SE ( ). * vs. the control group. # vs. the UPEC group. n p 6 P ! .05 P ! .05

Article Snippet: The human pyelonephritogenic E. coli strain J96 serotype O4:K6 (ATCC 700336), which expresses type 1 and P-fimbrial adhesions, was used.

Techniques: Isolation, Control

Journal: The Journal of infectious diseases

Article Title: Uropathogenic Escherichia coli alters muscle contractions in rat urinary bladder via a nitric oxide synthase-related signaling pathway.

doi: 10.1086/509620

Figure Lengend Snippet: Figure 3. Western-blot analysis of inducible nitric oxide synthase (iNOS) protein expression in the urinary bladder and kidney after the intravesical instillation of uropathogenic Escherichia coli (UPEC) or E. coli lipopolysaccharide (LPS). Urinary bladder and kidney were isolated from rats 24 h after the intravesical instillation of J96 or E. coli isolated from patients with acute E. coli pyelonephritis (EC) or LPS in the presence or absence of aminoguanidine (AG). a-tubulin served as a control (C) for sample loading and integrity. Quantification of the expression of iNOS protein was performed by densitometric analysis. Data are the mean SE ( ). * vs. the UPEC or LPS group. n p 6 P ! .05

Article Snippet: The human pyelonephritogenic E. coli strain J96 serotype O4:K6 (ATCC 700336), which expresses type 1 and P-fimbrial adhesions, was used.

Techniques: Western Blot, Expressing, Isolation, Control

Journal: The Journal of infectious diseases

Article Title: Uropathogenic Escherichia coli alters muscle contractions in rat urinary bladder via a nitric oxide synthase-related signaling pathway.

doi: 10.1086/509620

Figure Lengend Snippet: Figure 4. Expression of inducible nitric oxide synthase (iNOS) in the urinary bladder after the intravesical instillation of uropathogenic Esch- erichia coli (UPEC). Urinary bladders were isolated from rats 3 or 24 h after the intravesical instillation of J96 or E. coli isolated from patients with acute E. coli pyelonephritis (EC). In some experiments, rats were instilled intravesically with aminoguanidine (AG) or PD98059 (PD) 30 min after the instillation of UPEC. A, Representative typical Western blots of iNOS protein in the bladder isolated from rats 24 h after UPEC treatment. a-tubulin served as a control (C) for sample loading and integrity. B, top, Typical gel electrophoresis of reverse transcription–polymerase chain re- action of iNOS mRNA in bladders isolated from rats 3 h after UPEC treatment. The mRNA of b-actin served as a control for sample loading and integrity. Results shown are representative of at least 4 experiments.

Article Snippet: The human pyelonephritogenic E. coli strain J96 serotype O4:K6 (ATCC 700336), which expresses type 1 and P-fimbrial adhesions, was used.

Techniques: Expressing, Isolation, Western Blot, Control, Nucleic Acid Electrophoresis, Reverse Transcription

Journal: The Journal of infectious diseases

Article Title: Uropathogenic Escherichia coli alters muscle contractions in rat urinary bladder via a nitric oxide synthase-related signaling pathway.

doi: 10.1086/509620

Figure Lengend Snippet: Figure 5. Effects of uropathogenic Escherichia coli (UPEC) or lipopoly- saccharide (LPS) on the stimulation of extracellular signal-regulatedkinase (ERK)–1/2 mitogen-activated protein kinase (MAPK) in the urinary bladder. Urinary bladders were isolated from rats 24 h after the intravesical in- stillation of E. coli isolated from patients with acute E. coli pyelonephritis (EC) or with J96 or LPS in the presence or absence of aminoguanidine (AG) or PD98059 (PD). For measurement of the ERK1/2 phosphorylation and protein expression, Western-blot analysis was performed using anti– phospho (p)–ERK1/2 MAPK and anti-ERK1/2 MAPK antibodies. A, Rep- resentative typical Western blots of ERK1/2 in bladder. B, Analysis of the relative amounts of pERK1 in bladders treated with UPEC, relative to control. Data are mean SE ( ). * vs. control group. n p 5 P ! .05 # vs. UPEC groups. P ! .05

Article Snippet: The human pyelonephritogenic E. coli strain J96 serotype O4:K6 (ATCC 700336), which expresses type 1 and P-fimbrial adhesions, was used.

Techniques: Isolation, Phospho-proteomics, Expressing, Western Blot, Control

Journal: The Journal of infectious diseases

Article Title: Uropathogenic Escherichia coli alters muscle contractions in rat urinary bladder via a nitric oxide synthase-related signaling pathway.

doi: 10.1086/509620

Figure Lengend Snippet: Figure 6. Distribution of protein kinase C (PKC) isoforms between the cytosolic and membrane fractions in the urinary bladder. Urinary bladders were isolated from rats 24 h after the intravesical instillation of Esch- erichia coli isolated from patients with acute E. coli pyelonephritis (EC) or with lipopolysaccharide (LPS) in the presence or absence of amino- guanidine (AG). For measurement of the distribution of PKC isoforms, Western-blot analysis was performed using anti-PKCa and anti-PKCd antibodies. Quantification of the PKC isoforms was performed by den- sitometric analysis. Data are presented as PKC particulate:cytosolic ratio with means SEs ( ). * vs. control group. # vs. LPS n p 5 P ! .05 P ! .05 group. C, control.

Article Snippet: The human pyelonephritogenic E. coli strain J96 serotype O4:K6 (ATCC 700336), which expresses type 1 and P-fimbrial adhesions, was used.

Techniques: Membrane, Isolation, Western Blot, Control

Journal: The Journal of infectious diseases

Article Title: Uropathogenic Escherichia coli alters muscle contractions in rat urinary bladder via a nitric oxide synthase-related signaling pathway.

doi: 10.1086/509620

Figure Lengend Snippet: Figure 7. Effects of the intravesical instillation of uropathogenic Esch- erichia coli (UPEC) on detrusor contractions evoked by electrical field stimulation (EFS) or the exogenous agonist acetylcholine (ACh). Detrusor muscle strips isolated from rats 24 h after intravesical instillation of J96 or Escherichia coli isolated from patients with acute E. coli pyelonephritis (EC) were contracted by EFS (10 s at 4 and 16 Hz) (A) or ACh (3 mmol/ L) (B). In some experiments, rats were instilled intravesically with ami- noguanidine (AG) or PD98059 (PD) 30 min after the instillation of UPEC. Data are mean SE ( ). * vs. control group. # vs. n p 6 P ! .05 P ! .05 UPEC groups.

Article Snippet: The human pyelonephritogenic E. coli strain J96 serotype O4:K6 (ATCC 700336), which expresses type 1 and P-fimbrial adhesions, was used.

Techniques: Isolation, Control

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: In silico design and validation of a highly degenerate primer pair: a systematic approach

doi: 10.1186/s43141-020-00086-y

Figure Lengend Snippet: In silico PCR analysis of primer pair catAf/catAr with targeted sequences

Article Snippet: Klebsiella quasipneumoniae strain ATCC 700603 isolate K6 , NZ_CP014696.2 , 365 , 308-672 , 24.0.

Techniques: In Silico

Journal: Scientific Reports

Article Title: Probiotics: their action against pathogens can be turned around

doi: 10.1038/s41598-021-91542-3

Figure Lengend Snippet: The probiotic-pathogen shared gene orthogroups (genetic niche overlap) that might rationalize the benefit or detriment of the probiotic to the pathogen.

Article Snippet: Lactip. plantarum strain L75a & V. parahaemolyticus strain 64 , Single , Quorum sensing [0009372] , Autoinducer-2 production protein LuxS [ luxS ] , Lactip. plantarum ATCC BAA-793 [100%], V. parahaemolyticus O3:K6 strain RIMD 2210633 [99.419%].

Techniques: